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E2F4兔單克隆抗體

E2F4兔單克隆抗體

  • 價格￥2200
  • 規(guī)格100ul
  • 貨號HKZ150402
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150402E2F4兔單克隆抗體100ul支2200
E2F4
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50279?Clone# ?BP6256
Predicted?Molecular Wt:
44kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q16254
Background:
Transcription activator that?binds?DNA cooperatively with?DP?proteins through the?E2?recognition?site,?5′-TTTC[CG]CGC-3′ found?in the?promoter?region of a?number of genes whose?products?are?involved?in?cell?cycle?regulation?or?in?DNA?replication.?The?DRTF1/E2F?complex?functions?in?the?control?of?cell-cycle?progression?from?G1?to?S?phase.?E2F-4?binds?with?high?affinity?to RBL1 and?RBL2.?In?some?instances, can?also?bind?RB?protein.
E2F4 was considered a?potential?prognostic factor for?HNSCC.?Immunohistochemical staining?showed?that?E2F4?was?mainly?localized?in the cell?nucleus;?it was?highly expressed?in?HNSCC tissues, with?a?significant?difference?noted?from?that?in pericancerous?mucosa tissues.
Subcellular?location:
Nucleus
Recommended?Method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide. This?information?is?proprietary to?Biolynx.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage Conditions:
-25°C?to?-18°C
Shipment?Instructions:
Shipped?on?blue?ice.?Upon?delivery?store?at?-25°C?to?-18?°C. Avoid?freeze?/ thaw?cycles.
Recommended?Dilution:
IHC-P:?????1:100-1:200
Background?References:
1. ?I?Molina-Privado1, et?al.?Leukemia?(2012)?26,?2277-?2285.
2. ?Li?Qi, et?al. Scientifc?Reports(2022)?12:12132.
Immunohistochemistry?(Formalin/PFA-fixed?paraffin-??embedded?sections)?analysis of human?breast?cancer?labelling?E2F4 with?BP6256.

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