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Bcl-6兔單克隆抗體

Bcl-6兔單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150362
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150362Bcl-6兔單克隆抗體100ul1000
Bcl-6
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50239?Clone# ?BP6216
Predicted?Molecular Wt:
79kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P41182
Background:
Bcl-6?is a transcriptional?regulator gene which codes for?a?706-amino-acid?nuclear?zinc finger?protein.The?mitogen-activated
protein?kinases,?Erk1 and?Erk2,?but?not?JNK,?phosphorylate?BCL6 at?multiple?sites.?Phosphorylation?of?BCL6?at?Ser333?and
Ser343?results?in?degradation?of?BCL6?by?the?ubiquitin/proteasome?pathway?in?B?cells.?In?addition,?BCL6?is?acetylated?and?its
transcriptional?repressor function?is?inhibited?by the transcriptional co-activator?p300. Antibodies to this?protein stain?the
germinal center cells?in?lymphoid follicles, follicular cells?and?interfollicular?cells?in?Follicular?Lymphoma,?Diffuse?Large?B-Cell
Lymphomas,?Burkitt’s?Lymphoma, and the?majority of the?Reed-Sternberg cells?in?Nodular?Lymphocyte-Predominant?Hodgkin’s?Disease.
Subcellular?location:
Nuclear
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Recombinant fragment within?Human?Bcl-6.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Shvarts, A. et?al.?(2002)?Genes?Dev?16,?681-6.
2. ?Bereshchenko,?O.R. et?al.?(2002)?Nat?Genet?32,?606-13.
Immunohistochemistry
(Formalin/PFA-fixed?paraffin-
embedded?sections)?analysis of?human?diffuse?large?B cell
lymphoma tissue?labelling?Bcl-6?with?BP6216.?Heat?mediated
antigen?retrieval?was?performed?using?Tris/EDTA?buffer?pH?9.0

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