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MGMT兔單克隆抗體

MGMT兔單克隆抗體

  • 價格¥1400
  • 規(guī)格100ul
  • 貨號HKZ150363
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150363MGMT兔單克隆抗體100ul1400
MGMT
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50240?Clone# ?BP6217
Predicted?Molecular Wt:
22kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P16455
Background:
Involved?in the cellular defense against the?biological?effects?of?O6-methylguanine?(O6-MeG)?in?DNA.?Repairs?alkylated?guanine?in?DNA?by stoichiometrically transferring the alkyl group at?the?O-6?position?to?acysteine?residue?in?the?enzyme.?This?is?a
suicide?reaction: the enzyme?is?irreversibly?inactivated.
Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
A synthetic?peptide corresponding to?residues on the
N-terminus?of?human?MGMT?was?used?as?an immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Bobola,?M.S.?et?al.?(2005) Clin.?Cancer?Res.?11,?2747-2755.
2. ?Pollack,?I.F. et?al.?(2006) J. Clin.?Oncol.?24,?3431-3437.
Immunohistochemistry
(Formalin/PFA-fixed?paraffin-
embedded?sections)?analysis of?????human endometrial?cancer tissue?labelling?MGMT?with?BP6217.
Heat?mediated?antigen?retrieval?was?performed?using Tris/EDTA??buffer?pH?9.0

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