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VEGF兔單克隆抗體

VEGF兔單克隆抗體

  • 價格¥1000
  • 規(guī)格100ul
  • 貨號HKZ150359
  • 單位
  • 品牌HaoKebio

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品牌商品編碼商品名稱規(guī)格單位單價
HaoKebioHKZ150359VEGF兔單克隆抗體100ul1000
VEGF
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50236?Clone# ?BP6213
Predicted?Molecular Wt:
24kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P15692
Background:
Vascular?Endothelial Growth?Factor A?(VEGF-A; formerly VEGF)?belongs to the VEGF/PDGF family?of growth factors?that?play?a?critical?role?in?promoting vascular development. VEGF-A?is?primarily?known for?its function?in?promoting angiogenesis, the
process?by which?new?bloodvessels are sprouted from?pre-existing?bloodvessels. VEGF-A?has eight?isoforms,?derived?from
differentially spliced?mRNA variants encoded?by the VEGF-A gene. All?isoforms?contain?a?conserved?receptor?binding?domain,?but differ?in their C-terminal domains that?regulate?binding to?components?in?the?extracellular?matrix.?VEGF-A?is?secreted?by???multiple cell types,?including fibroblasts,?macrophages and some tumor?cells.?Binding?of VEGF-A to?its?cognate?receptors
(VEGFR2 and to a?lesser extent VEGFR1)?on?the?endothelial?cell?surface?activates the?receptor?and?initiates?downstream?signaling,?resulting?in endothelial cell?proliferation, survival,?migration, and?changes to?vascular?permeability,?all?crucial??processes for angiogenesis.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Recombinant?protein fragment of?human VEGF.
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Chaudhry?IH, O’Donovan?DG, et?al.?(2001)?.?Histopathology?91:409–415.
2. ?Boer?K, Troost?D, Spliet?WGM, et?al.(2008)?.?Acta?Neuropathol?115:?683–696.
Immunohistochemistry
(Formalin/PFA-fixed?paraffin-
embedded?sections)?analysis of?human?liver?carcinoma tissue
labelling?VEGF?with?BP6213.
Heat?mediated?antigen?retrieval?was?performed?using Tris/EDTA??buffer?pH?9.0

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