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OCT3/4兔單克隆抗體

OCT3/4兔單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150428
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150428OCT3/4兔單克隆抗體100ul1000
OCT3/4
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50305?Clone# ?BP6282
Predicted?Molecular Wt:
39kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q01860
Background:
OCT-4, also?known?as?OTF3 or?POU5F1,?is?a?member?of?the?POU?family?of?transcription?factors,?involved?in?the?regulation?of pluripotency during?normal development and?is detectable?in?embryonic?stem?and?germ?cells.?It?can?specifically?bind?to?the??octamer?motif?(5′-ATTTCAT-3′), and?it?is?critical?for?the?self-renewal?of?embryonic?stem?cells. OCT-4?is?a?key?regulator?of?self-?renewal?in embryonic stem cells;?its expression?is?potentially?correlated with?tumorigenesis?and?can?affect?some?aspects?of???tumor?behavior such as tumor?recurrence or?resistance to therapies.
OCT-4?is a sensitive and specific?marker for?germ?cell?tumors.?It?is?consistently?detected?in?carcinoma?in?situ/gonadoblastoma,
seminomas, germinoma, dysgerminoma, and embryonal?carcinoma?but?not?in the?differentiated?components?of?nonseminomas.
An antibody to OCT-4?is?useful?in the?identification of?primary?as?well?as?metastatic?germ?cell?tumors.
Subcellular?location:
Nucleus
Recommended?Method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide. This?information?is?proprietary to?Biolynx.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage Conditions:
-25°C?to?-18°C
Shipment?Instructions:
Shipped?on?blue?ice.?Upon?delivery?store?at?-25°C?to?–?18°C. Avoid?freeze?/ thaw?cycles.
Recommended?Dilution:
IHC-P:?????1:100-1:200
Background?References:
1.?Zhang?S??et?al.?Nucleic?Acids?Res?47:4449-4461?(2019).
2.?Chang?YC??et?al.?Cell?Death?Dis?11:195?(2020).
Immunohistochemistry?(Formalin/PFA-fixed?paraffin-embedded?sections)?analysis of ?human seminoma?tissue?labelling?OCT3/4???with?BP6282.

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