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Bcl-2鼠單克隆抗體

Bcl-2鼠單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150290
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150290Bcl-2鼠單克隆抗體100ul1000
BCL-2
Mouse?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50167?Clone#?8C8
Predicted?Molecular Wt:
26kDa
Purity: ??ProG?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P10415
Background:
BCL2?is?a?protein?associated?with?apoptosis?regulation?produced?by?the?bcl-2?gene,?located?on?chromosome?14q32.?BCL2 is ?comprised of an alpha?(239 amino?acids)?and?beta?chain.?BCL2?is found?in?mitochondrial?and?nuclear?membranes?and?in?the?cytosol?rather than the cell?surface.
In?normal?lymphoid tissue,?BCL2 antibody?reacts with small?B-lymphocytes?in the?mantle zone?and?many?cells?within?the?T-cell?areas. Anti-BCL2?has shown consistent?negative?reaction on?reactive germinal?centers?and?positive?staining?of?neoplastic
follicles?in follicular?lymphoma. This difference?in staining?pattern?is?not due to?down?regulation?or?decreased?BCL2?mRNA,?but?largely to a?post-translational?mechanism with?resultant decrease?in?protein?levels.
Consequently, this antibody?is valuable when distinguishing?between?reactive and?neoplastic follicular?proliferation?in??lymphoid?lesions. Anti-BCL2?has?been?used?as an?indicator of?minimal?residual?disease?in the?bone?marrow?of follicular?lymphoma?patients when staining?is strong and?uniform.
Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues?in?Human?BCL-2 was?used?as?an?immunogen.
PBS?59%, Sodium?azide?0.01%, Glycerol?40%,?BSA????????????
0.05%.
Storage conditions:
-20°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-20°C. Avoid?freeze?/ thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Gong?D??etal.?Mol?Ther?Nucleic?Acids?19:482-497?(2020).
2.?Wang?L??etal.?Proc?Natl?Acad?Sci?U?S?A?117:563-572?(2020).
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of tonsil?tissue?labelling?BCL-2 with?8C8.?Heat?mediated
antigen?retrieval was?performed?using
Tris/EDTA?buffer?pH?9.0

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