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Cytokeratin Pan兔單克隆抗體

Cytokeratin Pan兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150266
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150266Cytokeratin Pan兔單克隆抗體100ul600
Cytokeratin?Pan
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50143 Clone#??BP6051&BP6058
Predicted?Molecular Wt:
45-66kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P13645/P13647
Background:
The?keratins are the typical?intermediate filament?proteins of epithelia, showing?an?outstanding?degree?of?molecular?diversity.?Heteropolymeric filaments are formed?by?pairing of type?I and?type?II?molecules.?In?humans?54?functional?keratin?genes?exist.
They are expressed?in?highly specific?patterns?related to the?epithelial type?and?stage?of?cellular?differentiation.?This cocktail antibody can detect?both type?Icytokeratins?and??type?II?cytokeratin.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to Type?ICytokeratins?residues?within?aa100-200 of?Type?ICytokeratins?was ????used as?an?immunogen.
Synthetic?peptide corresponding to Type?II
Cytokeratins?residues?within?aa300-400 of?Type?II Cytokeratins was?used as an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Spagnolo?DV,?et?al. Am?J?Clin?Pathol.?1985?Dec;84(6):697-704.
2. ?Eichner?R, et?al. J?Cell?Biol.?1984 Apr;98(4):1388-96.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
esophagus tissue?labelling?Cytokeratin?Pan with
BP6051&BP6058.?Heat mediated?antigen
retrieval?was?performed????using Tris/EDTA?buffer?pH?9.0?

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