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CD4兔單克隆抗體

CD4兔單克隆抗體

  • 價(jià)格￥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150146
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150146CD4兔單克隆抗體100ul支600
CD4
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# BX50023?Clone# ?BP6028
?
Predicted?Molecular Wt:
51kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P01730
Background:
The CD4?molecule?is a glycoprotein,?belonging to?immunoglobulin superfamily?that?is?associated?with?HLA?class?II?antigen
recognition.?It?is a co-receptor that assists the T-cell?receptor?(TCR)?with?an?antigen-presenting?cell?and?also?interacts?directly?with?MHC class?II?molecules on the surface of the?antigen-presenting?cells?using?its?extracellular?domain.
In?lymphatic tissues, the CD4+ T cells are?seen?in?large?numbers?in the?parafollicular?zone,?while?scattered?cells?are?found?in?the???germinal centres and?mantle zone. CD4?is?also?demonstrated?in?hepatic?sinusoidal?cells,?monocytes?and?monocytes-derived?cells?but?not expressed on?B cells and?immature thymocytes.?Most?mature?T-cell?lymphomas?are?CD4?positive?with?the?exception?of
aggressive?NK-cell?leukaemia and extranodal?NK/T-cell?lymphomas: subcutaneous?panniculitis-like T-cell?lymphoma?(which?is
usually?CD8?positive), enteropathy-type T-cell?lymphoma and?hepatosplenic T-cell?lymphoma. CD4?is also?expressed?in?histiocytic?sarcomas and?Langerhans cell?histiocytosis as well as?in?splenic?littoral?cell?haemangioma.
CD4?plays an?important?role?in the classification of?lymphocytes?in?inflammatory?lesions and?malignant?lymphomas.
?Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to CD4?residues
within?aa200-300 of?CD4 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Karandikar?NJ, et.al, Am?J?Clin?Pathol.?2002?May;117(5):819-25.
2. ?Beaty?MW, et.al, Am?J?Surg?Pathol.?2001?Sep;25(9):1111-20.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections?analysis of tonsil?tissue?labelling CD4?with???BP6028.?Heat?mediated ???antigen?retrieval was
performed?using Tris/EDTA?buffer?pH?9.0

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