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AMACR鼠單克隆抗體

AMACR鼠單克隆抗體

  • 價格¥1000
  • 規(guī)格100ul
  • 貨號HKZ150373
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150373AMACR鼠單克隆抗體100ul1000
AMACR
Mouse?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50250???Clone# ?BPM6227
Predicted?Molecular Wt:
42kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q9UHK6
Background:
AMACR?(P504S?protein, 48?kDa) plays?a?role?in?the?beta-oxidation?of?branched-chain?fatty?acids?and?their?derivatives.?AMACR is found?in?mitochondria and?peroxisomes of?numerous tissues such?as?prostate,?liver,?biliary tract,?kidney?and?lung.?Using
immunohistochemistry,?non-neoplastic?prostate show a weak or focal?staining?reaction?in?about?20%?of the?cases.?The?reactivity tends to decrease with age?but the?correlation?coefficient?is?low.
A diffuse staining?pattern for AMACR?has?been found?in?more than?90%?of?prostate?carcinomas?irrespective?of?Gleason?score.?Particularly foamy,?pseudohyperplastic and atrophic variants of?carcinoma?may?be?negative.?In?prostatic?intraepithelial
neoplasia?(PIN), the?positive?rate?of?AMACR?ranges?from?13% to?72%.
AMACR antibody?is?mainly?used?in clinical diagnosis of?prostate?cancer.
Subcellular?location:
Cytoplasm
Recommended?Method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues of AMACR?was?used as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage Conditions:
-25°C?to?-18°C
Shipment?Instructions:
Shipped?on?blue?ice.?Upon?delivery?store?at?-25°C?to?–?18°C. Avoid?freeze?/ thaw?cycles.
Recommended?Dilution:
IHC-P:?????1:100-1:200
Background?References:
1. ?Marx, A. et?al.?(2008) Virchows?Arch?453,?243-248.
2. ?Li, W.?et?al.?(2008) J?Exp?Clin?Cancer?Res?27,?2.
Immunohistochemistry?(Formalin/PFA-fixed?paraffin-
embedded?sections)?analysis of?prostate cancer?labelling?AMACR?with?BPM6227.

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