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Glypican -3兔單克隆抗體

Glypican -3兔單克隆抗體

  • 價格￥1000
  • 規(guī)格100ul
  • 貨號HKZ150374
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150374Glypican -3兔單克隆抗體100ul支1000
Glypican-3
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50251?Clone# ?BP6228
Predicted?Molecular Wt:
66kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P51654
Background:
Glypican?3, also?known as GPC3,?is a?human?gene. The?protein?encoded?by?this?gene?is?a?member?of the?glypican?family.?Cell?surface?heparan sulfate?proteoglycans are composed of a?membrane-associated?protein?core?substituted?with?a?variable
number of?heparan sulfate chains.?Members of the glypican-related?integral?membrane?proteoglycan?family?(GRIPS)?contain?a?core?protein anchored to the cytoplasmic?membrane via aglycosyl-phosphatidylinositol?linkage. These?proteins?may?play?a
role?in the control of cell?division?and?growth?regulation.
Glypican-3 antibody?has?been?identified to?be a?useful tumor?marker for the?diagnosis?of?Hepatocellular?Carcinoma?(HCC),
Hepatoblastoma,?Melanoma, Testicular Germ Cell Tumors, and Wilms Tumor.?In?patients?with?HCC,?GPC3?was?overexpressed?in?neoplastic?liver tissue and elevated?in serum?but was?undetectable?in?normal?liver,?benign?liver,?and?the?serum?of?healthy
donors. GPC3 expression was also found?to?be?higher?in?HCC?liver?tissue?than?incirrhotic?liver?or?liver?with?focal?lesions?such?as?dysplastic?nodules and areas of?hepatic adenoma?(HA) with?malignant?transformation.
Glypican-3 antibody?is?mainly?used?in clinical diagnosis of?hepatocellular?carcinoma.
Subcellular?location:
Cytoplasm
Recommended?Method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues of?Glypican-3 was?used as an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage Conditions:
-25°C?to?-18°C
Shipment?Instructions:
Shipped?on?blue?ice.?Upon?delivery?store?at?-25°C?to?-18?°C. Avoid?freeze?/ thaw?cycles.
Recommended?Dilution:
IHC-P:?????1:100-1:200
Background?References:
1.?Anatelli?F, et?al. Am?J?Clin?Pathol.?2008;130(2):219-23-8.
2.??Kandil?D,et?al. Cancer.?2007;111(5):316-22.
Immunohistochemistry?(Formalin/PFA-fixed?paraffin-
embedded?sections)?analysis of?hepatocellular carcinoma?labelling?Glypican-3 with?BP6227.

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