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LEF1兔單克隆抗體

LEF1兔單克隆抗體

  • 價(jià)格¥2200
  • 規(guī)格100ul
  • 貨號(hào)HKZ150231
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150231LEF1兔單克隆抗體100ul2200
LEF1
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50108?Clone# ?BP6113
Predicted?Molecular Wt:
44kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q9UJU2
Background:
Lymphoid?Enhancing?Factor?1?(LEF1)?is a transcription factor that?belongs to?the TCF/LEF family.
LEF1?participates as a?regulator?in Wnt?signaling?pathways.?LEF1?is?an?important factor?in?lymphopoiesis?and?is?expressed?normally?in T and?pro-B cells?but?not expressed?in?mature?B?cells.
Anti-LEF1?may?be?used as an aid for?differentiation?of chronic?lymphocytic?leukemia/small?lymphocytic?lymphoma?from?other?small?B cell?lymphomas.
Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within?aa100-200 of?LEF1 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Ji?Q??etal.?Nat?Commun?10:313?(2019).
2. ?Przybyl?J??et?al. Gynecol?Oncol?149:388-393?(2018).
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
tonsil?labelling?LEF1 with?BP6113.?Heat?mediated ??antigen?retrieval was
performed?using
Tris/EDTA?buffer?pH?9.0.

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