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- 價(jià)格¥600
- 規(guī)格100ul
- 貨號(hào)HKZ150161
- 單位支
- 品牌HaoKebio
產(chǎn)品介紹
產(chǎn)品參數(shù)
資料下載
品牌 | 商品編碼 | 商品名稱 | 規(guī)格 | 單位 | 單價(jià) |
---|
HaoKebio | HKZ150161 | PSA兔單克隆抗體 | 100ul | 支 | 600 |
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PSA
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# BX50038?Clone# ?BP6043
?
Predicted?Molecular Wt:
29kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P07288
Background:
Prostate-specific antigen?(PSA)?is a single-chain glycoprotein?of 237?amino?acids?containing?approximately?8%?carbohydrate.?It?is aserine?protease?produced almost exclusively?by?prostatic epithelial?cells.
?
Immunohistochemically?PSA?is expressed?in the?highly specialized apically-superficial?layer?of female?and?male secretory?cells?of the?prostate gland, and?is?readily?demonstrated?in adenocarcinomas of the?prostate?in?about?99%?of the?cases. There?is?a???correlation?between?malignancy grade and?intensity of staining,?high grade?carcinomas?displaying weaker?expression.?About??1% of?poorly differentiated carcinomas?have?been?negative for?PSA.
?
Due to the?high specificity of?PSA for?prostatic glandular?epithelium,?it?is very?useful?in?identifying?prostatic?carcinoma?in?the?prostate and?in the adjacent organs often?affected?by?epithelial?malignancies,?i.e.?rectum?and?urinary?bladder.?PSA?may?be????used?in?a?panel together with?NKX3.1 and?Prostein, which are?at?least?as?sensitive?and?slightly?more?specific than?PSA.
?
Subcellular?location:
Secreted
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?PSA?residues within?aa150-250 of?PSA?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?WardAM, et.al, Ann?Clin?Biochem?2001;?38:?633-651.
2.?ACS?Aguiar?, et.al,?Histol?Histopathol?(2000)?15:?131-?142.
Immunohistochemistry?(Formalin/PFA-fixedparaffin-embedded
sections analysis?of
prostate cancer?tissue?labelling?PSA with
BP6043.?Heat?mediated antigen?retrieval?was
performed?using
Tris/EDTA?buffer?pH?9.0
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