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Podoplanin兔單克隆抗體

Podoplanin兔單克隆抗體

  • 價格¥1000
  • 規(guī)格100ul
  • 貨號HKZ150228
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150228Podoplanin兔單克隆抗體100ul1000
Podoplanin
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50105?Clone# ?BP6110
Predicted?Molecular Wt:
17kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q86YL7
Background:
Podoplanin was first?identified as a transmembrane glycoprotein?in the?podocytes?of?rat?kidney.?Podoplanin?acts?as?receptor?for selectins?(which?mediate?inflammatory cell adhesion) and?is?involved?in?cell?maturation?and?migration?by?filopodia
formation?(via the downregulation of the activities of?small?Rho?family?GTPases).
Podoplanin?is found?in?lymphatic endothelium?(but?not?bloodvessel endothelium), fibroblasts, osteocytes, follicular?dendritic??cells, smooth and striated?muscle cells,?myoepithelial?cells,?Cajal?cells,?basal?cells?in?squamous?epithelium?(tonsil),?gastric?crypt?cells,?prostatic?basal cells,?immature Sertoli cells and foetal?gonocytes?(but?not the?corresponding?cells?of the?adult?testis),
renal glomerular?podocytes,?mesothelium?(particularly when?reactive), subsets of?lymphocytes, Schwann?cells,?glial?and?ependymal cells.
Podoplanin?is a very?useful?marker?in the differentiation?between?malignant?mesothelioma and?adenocarcinoma,?and the?identification of seminoma/dysgerminoma/IGCN.
Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within??aa1-100 of?Podoplanin?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Bassarova?AV, Am?J?Surg?Pathol.?2006 Jul;30(7):878-82.
2. ?Biermann?K,?Histopathology.?2006 Sep;49(3):290-7.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of?lung??labelling?Podoplanin with?BP6110.?Heat?mediated ???antigen?retrieval was
performed?using
Tris/EDTA?buffer?pH?9.0.?

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