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Cytokeratin 8&18兔單克隆抗體Cocktail

Cytokeratin 8&18兔單克隆抗體Cocktail

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150268
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150268Cytokeratin 8&18兔單克隆抗體Cocktail100ul600
Cytokeratin?8&18
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50145 Clone#??BP6005&BP6054
Predicted?Molecular Wt:
54/48kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P05787/P05783
Background:
Cytokeratin?18?is a type?Icytokeratin.?It?is, together with?its filament?partner cytokeratin?8,?perhaps the?most?commonly?found?products of the?intermediate filament gene family.
In some epithelial cell types, cytokeratin?8?and?cytokeratin?18?are?the?sole?keratins?present.?The?classical?example?is?the?liver,?with cytokeratin 8/cytokeratin?18?representing the characteristic and?only?keratin?pair?of?normal?hepatocytes.?The?same?is
true for other?highly specialized?parenchymatous epithelia such?asacinar?cells?of the?pancreas,?proximal?tubular?epithelial
cells of the?kidney, and certain endocrine?cells?such?as?pancreatic?islet?cells.?Furthermore,?cytokeratin?8/cytokeratin?18?occur?together?with?other?keratins—in?various?pseudostratified?(e.g.?respiratory) and?complex?(e.g.?glandular)?epithelia?and?in?the ??urothelium;?in these composite epithelial tissues, cytokeratin?8?and?cytokeratin18?are?often?most?prominent?in the?lumen-
lining cells.?Even?in?non-keratinizing stratified squamous epithelia,?cytokeratin?8?and?cytokeratin?18?may?be focally?expressed?in the?basal cell?layer, together with cytokeratin?19?and the?constitutive?stratified-epithelial?keratins.
In?regard to?malignant tumors, cytokeratin 8 and?cytokeratin?18?are?expressed?in?most?carcinomas?except?for?some?differentiated squamous cell carcinomas. Therefore, cytokeratin?8?and?cytokeratin?18?antibody?strongly?stain?most??adenocarcinomas,?hepatocellular carcinomas,?renal cell carcinomas, and?neuroendocrine?carcinomas.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding?to cytokeratin 8
residues?within?aa383‐C?terminal?of?cytokeratin?8?was used as?an?immunogen.
Synthetic?peptide corresponding to cytokeratin?18
residues?within?aa330-430 of?cytoketatin?18?was?used as an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:50-1:100
Background?References:
1.?Wiedenmann?B,?et.al.?Proc?Natl?Acad?Sci?U?S?A.?1986?May;83(10):3500-4.
2.?Quinn?B. Am?J?Surg?Pathol.?1998?May;22(5):550-6.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded sections)?analysis of?colon
tissue?labelling?Cytokeratin????8&18 with?BP6005&BP6054.?Heat?mediated?antigen
retrieval?was?performed????using Tris/EDTA?buffer?pH?9.0

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