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Ep-CAM兔單克隆抗體

Ep-CAM兔單克隆抗體

  • 價格¥600
  • 規(guī)格100ul
  • 貨號HKZ150174
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150174Ep-CAM兔單克隆抗體100ul600
EpCAM
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50051?Clone# ?BP6056
Predicted?Molecular Wt:
35kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P16422
Background:
Epithelial cell adhesion?molecule?(EpCAM)?is a transmembrane glycoprotein,?may?act?as?a?physical?homophilic?interaction?molecule?between?intestinal epithelial cells?(IECs) and?intraepithelial?lymphocytes?(IELs) at?the?mucosal?epithelium for
providing?immunological?barrier as a first?line of defense?against?mucosal?infection.?It?plays?a?role?in?embryonic?stem?cells?proliferation and differentiation, and?it?up-regulates the expression?ofFABP5,?MYC?and?cyclins?A?and?E.
This glycoprotein?is?located on the cell?membrane surface?(preferentially?basolaterally)?and?in the?cytoplasm?of virtually?all
epithelial cells with the exception of?most?squamous?epithelia,?hepatocytes,?renal?proximal tubular?cells,?gastric?parietal?cells?and?myoepithelial cells.?However, focal?positivity?may?be?seen?in the?basal?layer of?squamous?cell?epithelium?of?endoderm
(e.g.,?palatine?tonsils) and?mesoderm?(e.g.,?uterine?cervix).?Normal?mesothelial?cells?are?EpCAM?negative,?but?may?express focal?reaction when?undergoing?reactive changes.?EpCAM?has?been seen?in the?majority of?epithelial?neoplasms,?whereas??most?non-epithelial?neoplasms do?not show?EpCAM expression.
In conjunction with other?markers,?EpCAM can?be?used?as?an?aid?in?determining?neoplasms?of?epithelial?origin,?such?as?distinguishing?between?lung adenocarcinoma and?mesothelioma.
Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?EpCAM?residues?????within?aa1-100 of?EpCAM?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Latza?et?al. J?Clin?Pathol.?1990;43:213-19.
2.?Ordonez?NG?et?al. Am?J?Clin?Pathol?1998;109(1):85-89.
Immunohistochemistry?(Formalin/PFA-fixedparaffin-embedded
sections?analysis of?colon?tissue?labelling?EpCAM
with?BP6056.?Heat mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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