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Type II Cytokeratins兔單克隆抗體

Type II Cytokeratins兔單克隆抗體

  • 價格￥600
  • 規(guī)格100ul
  • 貨號HKZ150176
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150176Type II Cytokeratins兔單克隆抗體100ul支600
Type?II Cytokeratins
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50053?Clone# ?BP6058
Predicted?Molecular Wt:
66/65/64/57/62/60/51/54kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Background:
The?keratins are the typical?intermediate filament?proteins of epithelia, showing?an?outstanding?degree?of?molecular?diversity.?Heteropolymeric filaments are formed?by?pairing of type?I and type?II?molecules.?In?humans?54?functional?keratin?genes?exist.???They are expressed?in?highly specific?patterns?related to the?epithelial type?and?stage?of?cellular?differentiation.
This?antibody?can?detect?high?molecular?weight?CK1, CK2,?CK3,?CK4,?CK5,?CK6,?CK7 and?CK8.?It?is?usually?used?in?a?cytokeratin cocktail with type?Icytokeratins antibody.
Subcellular?location:Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to Type?II Cytokeratins?residues?within?aa300-400 of?Type?II?Cytokeratins?was used as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Spagnolo?DV,?et?al. Am?J?Clin?Pathol.?1985?Dec;84(6):697-704.
2. ?Eichner?R, et?al. J?Cell?Biol.?1984 Apr;98(4):1388-96.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections?analysis of?liver?tissue?labelling Type?II?????Cytokeratins with
BP6058.?Heat?mediated antigen?retrieval?was
performed?using
Tris/EDTA?buffer?pH?9.0

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