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CD10兔單克隆抗體

CD10兔單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150177
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150177CD10兔單克隆抗體100ul1000
CD10
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# BX50054?Clone# ?BP6059
Predicted?Molecular Wt:
86kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P08473
Background:
CD10?is a single-chain cell surface glycoprotein,?also?is?designated?as?common?acute?lymphoblastic?leukaemia?antigen?(CALLA),?neprilysin and?neutral endopeptidase.?Besides, CD10?is a?zink-dependent?peptidase?(metalloprotease),degrading various
bioactive?peptides, and?it?plays a functional?role?by?modulating cellular?responses to?peptide?substrates.
CD10?is?present on the cell surface of?bone?marrow?stem?cells?and?myelopoietic?cells?(including?neutrophils),follicular?centre?cells, few?mature?B-lymphocytes, and a subpopulation?of?parafollicular T-lymphocytes.?CD10?is?also found?in?enterocytes?in????the?upper?part of the?intestinal tract?(brush?border),in?liver?(bile canaliculi),?kidney?(glomerular and?proximal tubular?cells),????pulmonary alveolar cells,?myoepithelial cells of?breast?and?sweat?and?salivary?glands,?prostate glandular?cells,?placental
trophoblastic cells, endometrial stromal cells,?some?endothelial?cells,?and?a?minority?of?(myo-)fibroblasts?(including?skin????periadnexal cells). CD10?is expressed?in a?high?percentage?of?cases?of?acute?lymphoblastic?leukemia,?follicular?lymphoma,?Burkitt’s?lymphoma, some?hematopoietic tumors, and chronic?myelogenous?leukemias?in?lymphoid?blast?crisis.
CD10?is?particularly?useful?in the classification of?B-cell?leukaemias/lymphomas and classification?of?carcinomas?(identification
of?hepatocellular carcinoma and?renal cell carcinoma).?CD10?may?be?used?in?the?identification?of?metaplastic?breast
carcinoma,?prognostication of?breast carcinoma, and classification?of?uterine?mesenchymal?neoplasms?(identification?of?stromal sarcoma).
Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
A?recombinant?protein corresponding to CD10?residues?within?aa1-500 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Chu?P, et?al. Am?J?Clin?Pathol?2000?Mar;113(3):374-82.
2. ?Iwaya?K,et?al. Virchows?Arch?2002 Jun;440(6):589-93.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of tonsil?tissue?labelling?CD10
with?BP6059.?Heat mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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