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CD99兔單克隆抗體

CD99兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150131
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150131CD99兔單克隆抗體100ul600
CD99
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# ?BX50008?Clone# ?BP6013
?
Predicted?Molecular Wt:
19kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P14209
Background:
CD99?is?a?type?I?transmembrane?glycoprotein?encoded?by?the?CD99?gene?and?the?functions?of?CD99?in?cells?in?which?CD99?was highly expressed?have?been studied and they were?as?follows:?cell?death?of thymocytes?and?T?lymphocytes,?migration?through?monocyte endothelial junctions?by adhesion and diapedesis,?cell-cell?adhesion?in?lymphocytes,?maintenance?of?cellular
morphology?in?Hodgkin and?Reed/Sternberg cells, and?recruitment of T?cells.
?
CD99 expression?has?been?reported?in?many cell types, such ashematopoietic?cells,?endothelial?cells,?central?nervous?system????ependymal cells, thymocytes, granular cells of the?ovary,?Sertoli?cells,?and?pancreatic?islet?cells.?And?in?tumors?it?expressed?by???virtually almost all?Ewings sarcoma and?primitive?peripheral?neuroectodermal tumors?(ES/PNET)?and?demonstrates?strong?and?di?use?membranous staining. Other tumors that?mayshow CD99 expression?include?neuroendocrine?carcinomas,
mesenchymal chondrosarcomas, solitary fibrous tumors, synovial sarcomas,?vascular?tumors,?small?round?blue?cell?tumors,?lymphoblastic?lymphoma, acute?myeloid?leukemia, and?myeloid sarcoma.
?
Studies?have shown that CD99?may?be a sensitive?marker?for?Ewing’s?sarcoma?and?peripheral?neuroectodermal?tumors?and
?

Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to CD99?residues?within?aa85‐C?terminal?of?CD99?was?used?as?an
immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of?human?Ewing’s sarcoma?tissue
labelling?CD99 with?BP6013.?Heat?mediated?antigen
retrieval?was?performed????using Tris/EDTA?buffer?pH?9.0

Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Gawon?Choi, et?al. Journal?of?Pathology?and Translational?Medicine?2016; 50:?361-368.
2.?Ventura?S, et?al.?Oncogene.?2016 Jul?28;35(30):3944-54.

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