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Vimentin兔單克隆抗體

Vimentin兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150129
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150129Vimentin兔單克隆抗體100ul600
Vimentin
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# ?BX50006?Clone# ?BP6010
?
Predicted?Molecular Wt:
54kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P08670
Background:
Vimentin?is the?most common?member of intermediate filament?(IF) family?and?one?of the?main?components?in?cytoskeleton
structure.?It?is essential?in the?role of cell?integrity and?cytoskeletal?stability. The?reorganization?of?vimentin,?similar?to?all?IF
proteins, occurs during different stages?of the?cell?cycle?and?cell?signaling?by?a?site-specific?phosphorylation?(serine?and?threonine?residues).
?
Vimentin?is expressed?in a wide variety of?mesenchymal?cell?types:?fibroblasts,?endothelial?cells?etc.,?and?in?a?number?of?other?cell
types derived from?mesoderm, e.g.,?mesothelium and?ovarian granulosa?cells.?However,?in?non-vascular?smooth?muscle?cells,?vimentin?is often?replaced?by?desmin.?In striated?muscle, vimetin?is also?replaced?by?desmin.?However, during?regeneration,????vimentin?is?reexpressed. Cells of the?lymfo-haemopoietic system?(lymphocytes,?macrophages etc.)?also?express vimentin,
sometimes?in scarce amounts.?In tumor tissues,?it?is?present?in?many different?neoplasms?but?is?particulary?expressed?in?those?originated from?mesenchymal cells.
?
In combination with a?panel of?antibodies,?it?is?used to?identify?tumor?with?mesenchymal?origin?and?malignant?melanoma.?Additionally, vimentin?is a?useful control?marker for?proper tissue?processing.
?

Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to Vimentin?residues
within?aa366‐466 of?Vimentin?was?used?as?an immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded?sections)?analysis of?human?colon
tissue?labelling vimentin
with?BP6010.?Heat?mediated antigen?retrieval?was
performed?using Tris/EDTA?buffer?pH?9.0
Background?References:
1.?Xiaotian?Jia, et?al,Oncol?Lett.?2016 Sep;?12(3):?1717–?1720.
2. ?Bo?Su, et?al,Oncotarget.?2016?Mar?1;?7(9):?10498–?10512.

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