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p16鼠單克隆抗體

p16鼠單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150384
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150384p16鼠單克隆抗體100ul1000
p16
Mouse?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50261???Clone# ?BPM6238
Predicted?Molecular Wt:
17kDa
Purity: ??ProG?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P42771
Background:
The?progression of cells through the cell cycle?is?regulated?by?a?family?of?protein?kinases?known?as?cyclin-dependent?kinases?(Cdks). The sequential activation of individual?members?of this?family?and?their?consequent?phosphorylation?of?critical
substrates?promotes orderly?progression through the cell cycle.??Moreover, the?gene?encoding?p16?INK4A?exhibits?a?high?frequency of?homozygous deletions and?point?mutations?in established?human?tumor?cell?lines.
p16?INK4A?expression?is?commonly?inhibited?by?lack?of?stress?and?is?thought?to?promote?cellular?senescence.?And?p16 INK4A?expression?is a commonly?used cellular?marker of?senescence.
p16?INK4A?protein expression?is?normally?altered?in?human?cancer cells, and?its?high expression?can?be?used?as?a?predictive?biomarker?in cervical cancer?cells.
Subcellular?location:
Nuclues
Recommended?Method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Full?length?recombinant?p16?INK4A?of?human?origin was?used as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage Conditions:
-25°C?to?-18°C
Shipment?Instructions:
Shipped?on?blue?ice.?Upon?delivery?store?at?-25°C?to?-18?°C. Avoid?freeze?/ thaw?cycles.
Recommended?Dilution:
IHC-P:?????1:100-1:200
Background?References:
1. ?Romagosa,?C. et?al.?(2011) Oncogene?30,?2087-97.
2. ?LaPak,?K.M. and?Burd,?C.E.?(2014)?Mol?Cancer?Res?12,?167-83.
Immunohistochemistry?(Formalin/PFA-fixed?paraffin-embedded?sections)?analysis of cervical?intraepithelial?neoplasias ?(CIN)
labelling?p16 with?BPM6238.?

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