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COL4A5兔單克隆抗體

COL4A5兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150352
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150352COL4A5兔單克隆抗體100ul600
COL4A5
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50229?Clone# ?BP6206
Predicted?Molecular Wt:
161kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P29400
Background:
Type?IV collagen?is the?major structural component of?glomerular?basement?membranes?(GBM),?forming?a?‘chicken-wire’?meshwork together with?laminins,?proteoglycans and entactin/nidogen.
Deletions?covering?the?N-terminal?regions?of?COL4A5 and?COL4A6,?which?are?localized?in?a?head-to-head?manner,?are?found?in the chromosome Xq22.3 centromeric deletion syndrome. This?results?in?a?phenotype?with?features?of?diffuse?leiomyomatosis???and Alport syndrome?(DL-ATS).
Subcellular?location:
basement?membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Recombinant fragment with COL4A5. This?information?is?proprietary to?Biolynx and/or?its suppliers.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Tsugio?Seki?et?al.?Histochem?Cell?Biol?(1998)?110:359-366
2.?Yoshioka?K?etal. Am. J.?Pathol?(1994)?.?144: 986-996
Immunohistochemistry
(Formalin/PFA-fixed?paraffin-?embedded?sections)?analysis??of?human?kidney tissue
labelling?COL4A5 with?BP6206.
Heat?mediated?antigen
retrieval?was?performed?using?Tris/EDTA?buffer?pH?9.0

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