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IDH1 R132H兔單克隆抗體

IDH1 R132H兔單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150351
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150351IDH1 R132H兔單克隆抗體100ul1000
IDH1?R132H
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50228?Clone# ?BP6205
Predicted?Molecular Wt:
47kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??O75874
Background:
Heterozygous?point?mutations of?IDH1 codon?132 are frequent?in World?Health?Organization?(WHO)?grade?II?and?III?gliomas.?IDH1?R132H?mutations occur?in approximately 70% of?astrocytomas?and?oligodendroglial tumors.?The?high?frequency?and?????distribution of the?IDH1?R132H?mutation among specific?brain tumor?entities allow?the?highly?sensitive?and?specific
discrimination of various tumors?by?immunohistochemistry, such as anaplastic?astrocytoma?from?primary?glioblastoma?or
diffuse astrocytoma WHO grade?II from?pilocytic astrocytoma?or?ependymoma.?Noteworthy?is the?discrimination?of the infiltrating edge of tumors with?IDH1?mutation from?reactive gliosis. This?antibody?is?highly?useful
for?tumor?classification?and?in detecting single?infiltrating tumor cells. The?routine?practical?approach for?diagnosing?astrocytomas?and?oligodendrogliomas?begins?with?perfoming?IHC?for?IDH1?R132H?and?ATRX?expression.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide. This?information?is?proprietary to?Biolynx and/or?its suppliers.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Reuss?D?etal.Acta?Neuropathol.?129(1):133-146,?2015
2. ?David?NL?etal. Acta?Neuropathol.?131:803-820,?2016
Immunohistochemistry
(Formalin/PFA-fixed?paraffin-?embedded?sections)?analysis??of?human??glioma?(IDH1
R132H) ?tissue?labelling?IDH1?R132H?with?BP6205.?Heat
mediated?antigen?retrieval?was?performed?using
Tris/EDTA?buffer?pH?9.0

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