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ZAP-70兔單克隆抗體

ZAP-70兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150224
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150224ZAP-70兔單克隆抗體100ul600
ZAP-70
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50101?Clone# ?BP6106
Predicted?Molecular Wt:
70kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P43403
Background:
Zeta-associated?protein-70?(ZAP-70)?is a?member of the Syk family of tyrosine?kinases,?a?group?of?proteins?that?attach?to?the
zeta chain componentsofT-cell?receptors to signal downstream?events?involved?in?the?regulation?of?cell function,
proliferation, and?death.?Research suggests that the ZAP-70?protein?may?also?play?an?important?role?in?natural?killer?(NK)?cell???activation?and early?B-cell development;?however,?it?is?not expressed?in?most?normal?mature?B-cells.?Expression of?ZAP-70?has?been?reported?in various?lymphomas,?including?mantle cell?lymphoma, small?lymphocytic?lymphoma and?marginal?zone
lymphoma.?During thymocyte development, ZAP-70?promotes survival and?cell-cycle?progression?of?developing thymocytes?before?positive selection?(when cells are still CD4/CD8?double?negative).?Additionally,?ZAP-70-dependent?signaling?pathway?may also contribute to?primary?B-cells formation and?activation through?B-cell?receptor?(BCR).
Subcellular?location:
Nuclear and?cytoplasmic
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within
aa200-350 of?ZAP-70 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA
0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store
at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:IHC-P:?????1:100-1:200
Background?References:
1.?Crespo, et?al.N?Engl?J?Med.?2003?May?1;348(18):1764-75.
2. ?National Committee for Clinical?Laboratory Standards(NCCLS).?Villanova,?PA?1991;7(9). Order?code?M29-P.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded?sections analysis
of ?tonsil tissue?labelling?ZAP-70 with?BP6106.
Heat?mediated?antigen
retrieval?was?performed
using Tris/EDTA?buffer
pH?9.0

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