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E-Cadherin兔單克隆抗體

E-Cadherin兔單克隆抗體

  • 價(jià)格¥1125
  • 規(guī)格100ul
  • 貨號(hào)HKI0391A
  • 單位
  • 品牌HaoKebio

產(chǎn)品介紹
產(chǎn)品參數(shù)
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品牌商品編碼商品名稱規(guī)格單位單價(jià)
HaoKebioHKI0391AE-Cadherin兔單克隆抗體100ul1125

Applications

Tested Applications:

FC, IF, IHC, WB,ELISA


Cited Applications:

FC, IF, IHC, WB

Species Specificity:

human, pig, rat


Cited Species:

human, monkey, mouse, pig


Positive WB detected in:

PC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells


Positive IHC detected in:

human breast cancer tissue, human colon tissue, rat colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:

human breast cancer tissue, human kidney tissue


Positive FC detected in:

A431 cells


Recommended dilution:

WB : 1:2000-1:8000

IHC : 1:1000-1:4000

IF : 1:200-1:800

Product Information


Source:

Mouse


Purification method:

Protein A purification


Isotype:

IgG2b


Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Full name:

cadherin 1, type 1, E-cadherin (epithelial)


Calculated molecular weight:

882 aa, 97 kDa


Observed molecular weight:

120 kDa



Gene symbol

CDH1


Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin

Background

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocols

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