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Phospho histone H3( PHH3)兔單克隆抗體

Phospho histone H3( PHH3)兔單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150210
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150210Phospho histone H3( PHH3)兔單克隆抗體100ul1000
Phosphohistone?H3 (PHH3)
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50087?Clone# ?BP6092
Predicted?Molecular Wt:
15kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P68431
Background:
Phosphohistone?H3?(PHH3)?is?a?marker?specific?for?cells?undergoing?mitosis. Serine?10?of?Histone?H3?is?phosphorylated?in association with?mitotic chromatin condensation?in?late G2?and?M?phase?of the?cell?cycle?and thus,?PHH3?can?distinguish??mitosis from apoptotic?nuclei.
The?range?of?percentage?PHH3?positive?tumor?nuclei?was?from?0.0 to?6.6%?(median?value?0.8%).?Increased?expression?of?PHH3?was?significantly?associated?with?tumor?thickness?(p?= 0.031),?presence?of?tumor?ulceration?(p?=0.041) and?tumor?necrosis?(p?=?0.027),?but?not?with?Clark’s?level?of?invasion.?High?levels?of?PHH3 was?associated?with?increased?mitotic?count?(p?=?0.003)?and ??high?Ki-67 expression?(p =?0.002).
For central?nervous system tumors,?melanoma, soft tissue tumors,?GIST,?etc.,?PHH3?mAb?is?helpful for?tumor?pathological?classification and?prognosis.
Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?Phosphohistone?H3?(PHH3)?residues?within?aa1-100?(phosphoS10) ?was
used as?an?immunogen.
?Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?TharejaS, et?al. Am?J?Dermatopathol. 2014 Jan;?36(1):64-7.
2.?Casper?DJ, et?al. Am?J?Dermatopathol.?2010 Oct;?32(7):650-4.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of tonsil
tissue?labelling
Phosphohistone?H3
(PHH3) with?BP6092.
Heat?mediated?antigen???retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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