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ALK兔單克隆抗體

ALK兔單克隆抗體

  • 價(jià)格￥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150310
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150310ALK兔單克隆抗體100ul支1000
ALK
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50187?Clone# ?BP6165
Predicted?Molecular Wt:
177kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q9UM73
Background:
Anaplastic?lymphoma?kinase?(ALK)?is a?receptor tyrosine?kinase of the?insulin?receptor superfamily. ALK?is typically?expressed?at?low?levels?in?regions of the developing central and?peripheral?nervous?system.
ALK?may?be activated?in cancer through?multiple?mechanisms. The?most common?mechanism?is through?formation?of?a?fusion
protein from chromosomal translocations, as?in the case?of?anaplastic?large?cell?lymphoma?(ALCL)?and?inflammatory
myofibroblastic tumors. ALK?may also?be amplified through?mutation, as?in?neuroblastomas.?Various?solid?tumors,?such?as?non-small cell?lung carcinoma?(NSCLC) and?brain cancers were?also?found?to?aberrantly?express?ALK.
ALK staining?is?present within?both the?nucleus and cytoplasm,?and?are?positive?in?about?60%?of ALCL.?ALK?protein?expression?by tumor cells?is an?independent?prognostic factor that?predicts a?favorable?outcome.
Subcellular?location:
Cytoplasm&Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Recombinant?protein of?Human ALK was?used as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Zhu?W??etal. Transl?Stroke?Res?(2018).
2.?Yang?Y??et?al.?Biol?Open?8:?(2019).
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis
of ?human?non-small?cell?lung carcinoma tissue
labelling ALK?with
BP6165.?Heat?mediated antigen?retrieval was
performed?using
Tris/EDTA?buffer?pH?9.0

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