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Myosin (Smooth Muscle)兔單克隆抗體

Myosin (Smooth Muscle)兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號HKZ150209
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150209Myosin (Smooth Muscle)兔單克隆抗體100ul600
Myosin?(Smooth?Muscle)
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50086?Clone# ?BP6091
Predicted?Molecular Wt:
227kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P35749
Background:
Smooth?Muscle?Myosin,?heavy chain?(SMM-HC)?is a cytoplasmic structural?protein that?is?a?major?component?of the
contractile apparatus of the smooth?muscle cells.?Expression?of smooth?muscle?myosin?is?developmentally?regulated,
appearing early and?is specific for smooth?muscle?development.?SM-MHC?stains?the?intact?myoepithelial?cell?(MEC)?layers????present?in?benign and?in situ?malignant?breast and?bronchioloalveolar?lesions and?is therefore?very?helpful?in?distinguishing?between?benign and?malignant tumors.
The antibody?reacts with smooth?muscle cells and?myoepithelial?cells,?but?not?with?myofibroblasts.??It?is?very?helpful?in
distinguishing?between?benign sclerosing?breast?lesions and?infiltrating carcinomas?in difficult?cases?since?it?strongly?stains the?myoepithelial?layer?in the?benign?lesions while?it?is?negative?in the?infiltrating carcinomas.Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?Myosin?(Smooth?Muscle)?residues?within?aa1-100 of??Myosin?(Smooth Muscle) was?used as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Hilson?JB, et?al. Am?J?Surg?Pathol.?2010 Jun;?34(6):896-900.
2. ?Nicolas?MM, et?al.?Hum?Pathol.?2010?May; 41(5):663-71
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis
of??liomyoma tissue
labelling?Myosin
(Smooth?Muscle) with????BP6090.?Heat?mediated antigen?retrieval?was
performed?using
Tris/EDTA?buffer?pH?9.0

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