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TdT兔單克隆抗體

TdT兔單克隆抗體

  • 價格￥1000
  • 規(guī)格100ul
  • 貨號HKZ150207
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150207TdT兔單克隆抗體100ul支1000
TdT
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50084?Clone# ?BP6089
Predicted?Molecular Wt:
58kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P04053
Background:
Terminal deoxynucleotidyl transferase?(TdT)?is an?unusual deoxynucleotide?polymerizing enzyme?with?a?molecular?weight?of???about?58?kDa found?normally only?in?B- and T-cell?lymphoblasts/prelymphocytes. TdT generates antigen?receptor?diversity?by?synthesizing?non-germline elements?(N-regions) at the junctions of?rearranged?Ig?heavy chain?and T?cell?receptor?gene
segments.Rare TdT-positive cells are?regularly detected?in thymus and?bone?marrow.?Typically,?TdT?expression?in?the?thymus?is
very variable from cell to cell since?it?is?rapidly?decreased?in?more?mature T-cells.?Tdt-positive?cells?may?occasionally?be?found?in tonsils,?lymph?nodes and extranodal?lymphoid tissue.?Immunohistochemical detection?of TdT?has value?in?classification?of???malignant?lymphomas and acute?leukaemias,?particularly for the?identification of?pre-B?and?pre-T?acute?lymphoblastic
leukemia/lymphoblastic?lymphoma?(ALL/LBL).
?Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within?aa1-100 of?TdT?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Chang?LM,?Bollum?FJ.?CRC?Crit?Rev?Biochem.?1986;21(1):27-52.
2. ?Kang?LC,?Dunphy?CH. Arch?Pathol?Lab?Med.?2006;130(2):153-7.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
thymoma?labelling TdT?with?BP6089.?Heat
mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0.

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