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S100兔單克隆抗體

S100兔單克隆抗體

  • 價格¥600
  • 規(guī)格100ul
  • 貨號HKZ150206
  • 單位
  • 品牌HaoKebio

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品牌商品編碼商品名稱規(guī)格單位單價
HaoKebioHKZ150206S100兔單克隆抗體100ul600
S100
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50083?Clone# ?BP6088
Predicted?Molecular Wt:
21kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P04271
Background:
S100?is a?21kDa?highly?acidic and water?soluble?protein, which?is?expressed?in?a?wide?variety?of?normal?and?neoplastic?cells?of?mesodermal,?neuroectodermal, and epithelial origin. The?protein?is a?dimer,?having?two?subunits,?alpha?and?beta,?with
extensive?sequence?homology. There?are?3 forms?of?S-100?protein:?alpha-alpha,?known?as?S-100A0;?alpha-beta,?known?as?S-
100A?and?beta-beta,?known?as?S-100B. The?immunohistochemical?evaluation?of?S-100?(beta)?protein?expression?is?important?in the diagnosis of?undifferentiated?malignant tumours of?unknown?primary origin?and?should?be?included?in?the?so-called
primary?panel. S100 is?a?very?sensitive?marker?for?malignant?melanoma?of?all?types.?S100?may?be?used?in?the?differential diagnosis of sarcomas and spindle?cell?tumours.Subcellular?location:
Cytoplasm,?Nuclears
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within?aa50-150 of?S100 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Hagen?EC, Vennegoor?C, Schlingemann?RO, Van?der Velde?EA,?Ruiter?DJ.?Histopathol?1986;10:689-700.
2.?Schafer?BW,?Heizmann?CW. Trends?Biochem?Sci.?1996?Apr;21(4):134-40.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
melanoma?labelling?S100?with?BP6088.?Heat
mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0.

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