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CD117兔單克隆抗體

CD117兔單克隆抗體

  • 價(jià)格￥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150182
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150182CD117兔單克隆抗體100ul支1000
CD117
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# BX50059?Clone# ?BP6064
Predicted?Molecular Wt:
110kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P10721
Background:
CD117?is?a?cell?membrane?protein?encoded?by?the?c-kit?proto-oncogene?(chromosome?4q11-12).?The?protein?is?a?type?III
tyrosine?kinase growth factor?receptor for stem cell factor?(SCF),?also?known?as?mast?cell growth?factor.?CD117?is?required?for?the development and growth of?a?large?number?of?cells?expressing this?protein.
CD117?is expressed?in?mast cells,?melanocytes and?interstitial cells?of?Cajal?(ICC). The?cells?(particularly?the?mast?cells)?show?a???strong?membrane as well as cytoplasmic staining.?CD117?moreover?is?expressed?in?various?epithelia?(breast,?sweat?glands?and
salivary glands,?renal tubular cells, thyroid follicular cells),?usually?showing?a?weaker,?cytoplasmic?reaction.?In?tumor?tissues,?CD117?is expressed?in?most cases of Gastrointestinal?stromal tumor?(GIST),?mast?cell?neoplasms,?malignant?melanoma
(however, the expression of CD117?is?lost during?progression?and?metastasising),seminoma/dysgerminoma?and?intratubular?germ cell?neoplasia, endometrial carcinoma, follicular and?papillary?thyroid?carcinoma,?Merkel?cell?carcinoma,?cylindroma,?????malignant glioma, and angiomyolipoma.CD117?may?be?useful for the?classification?of several?cancers?expressing?c-kit,
including gastrointestinal stromal tumors?(GISTs),?mast cell diseases,?acute?myeloid?leukemia?(AML),?small?cell?lung?carcinoma?(SCLC), and?Ewing’s?sarcoma.
Subcellular?location:
Membrane&Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to CD117?residues
within?aa876-976 of??CD117 was?used?as?an immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Arber?et?al.?Hum?Pathol?1998;?29(5):498-504.
2.?Ashman?LK?et?al.?Int?J?Biochem?Cell?Biol.?1999?Oct;31(10):1037-51.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
gastrointestinal stromal?tumor ?tissue?labelling
CD117 with?BP6064.?Heat mediated?antigen
retrieval was?performed????using Tris/EDTA?buffer?pH?9.0?

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