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Cytokeratin 6兔單克隆抗體

Cytokeratin 6兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150183
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150183Cytokeratin 6兔單克隆抗體100ul600
Cytokeratin?6
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50060?Clone# ?BP6065
Predicted?Molecular Wt:
60kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P48668
Background:
Cytokeratin?6?is an epidermis-specific type?I?keratin?involved?in wound?healing.?Involved?in the?activation?of follicular
keratinocytes after wounding, while?it does?not?play a?major?role?in?keratinocyte?proliferation?or?migration.?This?protein?always?paired?with?the?type-I?keratin?K16.
Cytokeratin?6?have?been?identified?in?epidermis only?in?samples from?plantar glabrous skin while?in?hairy?skin?this?keratin
appeared to?be absent?in?interfollicular epidermis?but were clearly?present?in?hair follicle?outer?rootsheath,?and?this?protein?also consistently expressed?in?non-keratinizing stratified squamous?epithelia. Among?tumors,?cytokeratin?6?is typically?and?????strongly expressed?in squamous cell carcinomas?of?different?sites,?preferentially?in?inner,?maturing?layers?of the tumor?cell?????nests.
Cytokeratin?6?is always?used with cytokeratin 5?and?is?a?useful?aid for?classification?of?epithelioid?mesotheliomas.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?Cytokeratin 6
residues?within?aa464-564 of??Cytokeratin?6?was?used as an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?R?Mollet?al.?Histochem?Cell?Biol?(2008)?129:705–733.
2. ?R?Mollet?al.?Cell, Vol.?31,?1?l-24,?November?1982.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of
esophagus?cancer tissue?labelling Cytokeratin?6????with?BP6065.?Heat
mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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