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CD163兔單克隆抗體

CD163兔單克隆抗體

  • 價格¥600
  • 規(guī)格100ul
  • 貨號HKZ150181
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150181CD163兔單克隆抗體100ul600
CD163
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50058?Clone# ?BP6063
Predicted?Molecular Wt:
125kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q86VB7
Background:
CD163?is a type?I?membrane?protein, and?is a?member of?the?hemoglobin?scavenger?receptor?cystein-rich?superfamily.?The?protein?is?involved?in the clearance of?hemoglobin-haptoglobin complexes and?is?considered?to?have?anti-inflammatory
functions.
CD163 expression?is?restricted to the?monocytic/macrophage?lineage.?It?is expressed?by all?circulating?monocytes?and?by?a?majority of tissue?macrophages, such as splenicdendrocytes,?alveolar?macrophages?and?Kupffer?cells?of the?liver.?It?is?not
present?in?macrophages?in the?mantle zone and some of the?germinal?center?cells?in?lymph?follicles,?nor?in?Langerhans?cells?and?interdigitating?reticulum cells.?In tumor tissues, CD163?is found?in almost?all?cases?of?acute?myeloid?leukemia?with
monocytoid differentiation and?in the?majority of cases?of?histiocytic?sarcoma,?littoral?cell?angioma,?Rosai-Dorfman?disease,
Langerhans cell?histiocytosis and typical and atypical?fibrous?histiocytoma.?It?is?also?expressed?in?some?cases?of?dermatofibrosarcoma?protuberans.
CD163 can?be?used to detect cells?of?monocytic?and?histiocyte?lineage?in?neoplastic?and?reactive?lesions.?It?has?been?shown to?be?more sensitive than?CD68 for the detection of?macrophages?and?monocytic?cells.?It?covers?a?similar,?but?not?identical,
spectrum?of?cells?as?CD68.
Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to CD163?residues?within?aa1056-1156 of??CD163?was?used?as?an
immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Garcia C, et al.?Appl?Immunohistochem?Mol?Morphol?2008,?16(5), 417-21.
2. ?Nguyen?TT, et?al. Am?J?Surg?Pathol?2005,?29(5),?617-24.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections?analysis of?liver?tissue?labelling?CD163
with?BP6063.?Heat
mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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