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Cytokeratin 18兔單克隆抗體

Cytokeratin 18兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150172
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150172Cytokeratin 18兔單克隆抗體100ul600
Cytoketatin?18
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog#BX50049?Clone# ?BP6054
Predicted?Molecular Wt:
48kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P05783
Background:
Cytokeratin?18?(also?named?keratin?18)?is?a?type?Icytokeratin.?It?is, together?with?its?filament?partner?keratin?8,?perhaps?the?most commonly found?products of the?intermediate filament gene family.
In some epithelial cell types,?keratin 8?and?keratin?18?are?the?sole?keratins?present.?The?classical?example?is?the?liver,?with?keratin?8/keratin?18?representing the characteristic and only?keratin?pair of?normal?hepatocytes.?The?same?is?true?for?other?highly
specialized?parenchymatous epithelia such asacinar cells of?the?pancreas,?proximal?tubular?epithelial?cells?of?the?kidney,?and
certain endocrine cells such as?pancreatic?islet?cells.?Furthermore,?keratin?8/keratin?18?occur together?with?other?keratins—in?various?pseudostratified?(e.g.?respiratory) and complex?(e.g. glandular) epithelia?and?in the?urothelium;?in?these?composite
epithelial tissues,?keratin 8 and?keratin18 are often?most?prominent?in?the?lumen-lining?cells.?Even?in?non-keratinizing?stratified?squamous epithelia,?keratin 8 and?keratin?18?may?be focally?expressed?in the?basal?cell?layer, together?with?keratin?19?and?the???constitutive stratified-epithelial?keratins.
In?regard to?malignant tumors,?keratin 8 and?keratin?18 are?expressed?in?most?carcinomas?except?for?some?differentiated
squamous cell carcinomas. Therefore,?keratin 8 and?keratin?18?antibody?strongly?stain?most?adenocarcinomas,?hepatocellular?carcinomas,?renal cell carcinomas, and?neuroendocrine carcinomas.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to cytokeratin?18
residues?within?aa330-430 of?cytoketatin?18?was?used as an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Wiedenmann?B,?et.al.?Proc?Natl?Acad?Sci?U?S?A.?1986?May;83(10):3500-4.
2.?Quinn?B. Am?J?Surg?Pathol.?1998?May;22(5):550-6.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections?analysis of?liver?tissue?labelling
cytokeratin?18 with
BP6054.?Heat?mediated antigen?retrieval was
performed?using
Tris/EDTA?buffer?pH?9.0

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