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Myo D1兔單克隆抗體

Myo D1兔單克隆抗體

  • 價格¥600
  • 規(guī)格100ul
  • 貨號HKZ150243
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150243Myo D1兔單克隆抗體100ul600
Myo?D1
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50120?Clone# ?BP6124
Predicted?Molecular Wt:
35kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P15172
Background:
MyoD1, one of the?MyoD family of?myogenic?helix-loop-helix transcription factors,?combined?with?myogenin,?plays?a?role?in?coordinating the?myogenic differentiation?pathway from the determination of?mesodermal?precursors?into?myoblasts,?the
differentiation of?myoblasts?into?myotubes, and finally the?maturation of?myotubes?into?skeletal?myofibers.?Normal?mature?????skeletal?muscle does?not express?MyoD1?protein.?MyoD1?is expressed?in?myoblasts?before differentiation?while?myogenin?has?post-differentiation functions. Anti-MyoD1?immunostaining?identifies cells committed to?myogenesis?in their earliest?phase,????thus,?it?is a?better?biomarker for?less differentiated?Rhabdomyosarcomas?(RMS)?.
RMS are the?most frequent?malignant soft tissue?neoplasms of?childhood.?While?better?differentiated?RMS?have?cross-
striations or?rhabdomyoblasts that allow for a?confident?morphologic?diagnosis,?less?differentiated?RMS?resemble?other?small?blue?round-cell tumors.
Studies suggest, anti-MyoD1?may?be?used together with anti-myogenin?and?anti-desminas?a?panel?of?markers?since?any?RMS?is virtually?never?negative for all three?markers simultaneously.
Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within
aa1-100 of??Human?Myo?D1 was?used?as?an immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Farup?J??et?al. J?Appl?Physiol?(1985)?119:1053-63?(2015).
2.??Hoedt?A??etal. J?Physiol?594:727-43?(2016).
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
Rhabdomyosarcomas
tissue?labelling?Myo?D1?with?BP6124.?Heat
mediated?antigen
retrieval was?performed?using Tris/EDTA?buffer????pH?9.0

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