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Cytokeratin 14兔單克隆抗體

Cytokeratin 14克隆抗體

  • 價格￥600
  • 規(guī)格100ul
  • 貨號HKZ150170
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150170Cytokeratin 14兔單克隆抗體100ul支600
Cytokeratin?14
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50047?Clone# ?BP6052
Predicted?Molecular Wt:
52kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P02533
Background:
Cytokeratin?14?is a?member of the type?I?keratin family of?intermediate filament?proteins.?It?always?pairs?with?the?type?II
keratin?K5 and form the?primary?keratin?pair of the?keratinocytes of?stratified?squamous?epithelia,?including the?epidermis?as?well as?mucosal?non-keratinizing stratified squamous epithelia.
Cytokeratin?14?is strongly expressed?in the?undifferentiated?basal cell?layer containing?the?stem?cells?and?are?down-regulated??in the differentiating suprabasal cell?layers. Otherwise,?in?the?widely?well?stratified?follicular?outer?rootsheath,?cytokeratin?14?is?uniformly expressed throughout all?layers. The expression?spectrum?of cytokeratin?14?in?tumors?corresponds?well?to?the
patterns?in?normal epithelia. Thus,?most squamous cell carcinomas?as?well?as?malignant?mesotheliomas?strongly?express this?keratin whereas?little, focal, or?no expression?is found?in?adenocarcinomas.
Cytokeratin?14?may?be a?useful?marker?in the differential diagnosis of?squamous?cell?carcinoma?from?other?epithelial?tumors.?Recent studies also?indicate that CK14 expression?in?breast?cancer?corresponded?with?poor?clinical?outcome?and?that?CK14
may?have diagnostic value?in the sub-classification?of?NSCLC.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to cytokeratin?14
residues?within?aa372-472 of?cytokeratin?14?was?used as an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Reis-Filho?JS, et.al. Virchows?Arch.?2003?Aug;443(2):122-32.
2. ?Moll?R, et.al.?Histochem?Cell?Biol.?2008 Jun;129(6):705-?33.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of
esophagus tissue
labelling?cytokeratin?14?with?BP6052.?Heat
mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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