PMS2兔單克隆抗體

價格￥2200
規(guī)格100ul
貨號HKZ150235
單位支
品牌HaoKebio

產品介紹

產品參數

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品牌	商品編碼	商品名稱	規(guī)格	單位	單價
HaoKebio	HKZ150235	PMS2兔單克隆抗體	100ul	支	2200

PMS2
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50112?Clone# ?BP6116
Predicted?Molecular Wt:
96kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P54278
Background:
Mismatch?repair?(MMR)?proteins?is a group of?nuclear enzymes,?which?in?all?proliferating?cells?participate?in?repair?of?base-
base?mismatch, that occur?during?DNA?replication.?Loss of?MMR?proteins?leads to an?accumulation?of?DNA?replication?errors?in?the?proliferating cells,?particularly?in areas of the genome with?short?repetitive?nucleotide?sequences,?a?phenomenon?known?????as?microsatellite?instability?(MSI).
The?PMS2?protein forms a?heterodimer with the?MLH1?protein which?is then?activated?in the?presence?of ATP;?this?complex???coordinates the?binding of other?proteins that?repair?DNA errors arising?during?cell?preparation?for?cell?division.?The?loss?of?????PMS2 expression?in tumors can?be?helpful?in?identifying?hMLH1?mutation carriers and?identifies?their?suitability?for?mutation
analysis.?PMS2 gene defects account for a?small?but?significant?proportion?of?colorectal?cancers?and?for?a?substantial?proportion of tumors with?microsatellite?instability.
Compared to?molecular?biological techniques,?immunohistochemical analysis of?MMR?protein expression?is?much?simpler?and?cheaper.?Immunohistochemical analysis?helps to?pinpoint the affected gene and?should?be?readily?accessible?in?a?pathology
laboratory.
Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within?aa1-100 of?PMS2 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Shia?J, Virchows?Arch.?2004?Nov;445(5):431-41.?Epub
2004 Sep?29.
2.?Wright?CL, Am?J?Surg?Pathol.?2003?Nov;27(11):1393-?406.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
esophagus?labelling
PMS2 with?BP6116.?Heat mediated?antigen
retrieval was?performed?using Tris/EDTA?buffer????pH?9.0.

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