一抗 抗體 試劑 Cyclin D1兔單克隆抗體一抗 抗體 試劑 價(jià)格￥1000規(guī)格100ul貨號HKZ150194單位支品牌HaoKebio QQ53383090 咨詢購買 產(chǎn)品介紹產(chǎn)品參數(shù)資料下載 品牌商品編碼商品名稱規(guī)格單位單價(jià)HaoKebioHKZ150194Cyclin D1兔單克隆抗體100ul支1000Cyclin?D1Recombinant?Rabbit?Monoclonal Antibody?Product?DatasheetCatalog# ?BX50071?Clone# ?BP6076Predicted?Molecular Wt:34kDaPurity: ??ProA?affinity?purified?IgGSpecies Cross-reactivity:HumanForm: ??LiquidApplications:IHC-PSwissprot?ID: ??P24385Background:Cyclin?D1?(synonymes:?PRAD1,?parathyroid?adenomatosis?1,CCND1) is?a?295 amino?acid?protein,?36?kDa,?which?belongs?to?thehighly conserved cyclin family, whose?members are?characterized?by?a?dramatic?periodicity?in?protein?abundance throughout?the cell cycle. Cyclin?D1 forms a?complex?with?and?functions?as?a?regulatory?subunit?of?CDK4?or?CDK6,?whose?activity?isrequired for cell cycle G1/S transition. The?protein?has?been?shown to?interact?with?tumour?suppressor?protein?Rb?andits?expression?is?regulated?positively?by?Rb.The expression?is cell cycle dependant,?maximal?in?G1?and?minimal?in?S?phase.?Localization?is?mainly?nuclear.?In?normal tissues,?Cyclin?D1 expression?is?restricted to the?proliferative zone of epithelial?tissues,?endothelium?and?some?fibroblasts.?There?is?no???expression?in?lymphoid tissue.Mutations with amplification of the cyclin?D1 gene,?with?overexpression?of the?protein,?which?alters?cell?cycle?progression,?are?observed frequently?in a variety of tumours and?may?contribute?to?tumourigenesis.In?diagnostic?pathology,?immunohistochemical detection of Cyclin?D1?is?used?mainly for the?diagnosis?of?mantle?celllymphoma. While other?methods of cyclin?D1 detection?are?being?explored,?immunohistochemistry?is?still?widely?used?and?anti-cyclin?D1 antibodies?remain an essential component?in?a?small?B-cell?lymphoproliferative?disorder?panel.Subcellular?location:NucleusRecommended?method:Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.Immunogen:Synthetic?peptide corresponding to?residues within???aa200-295 of??Cyclin?D1 was?used?as?an?immunogen.Storage?Buffer:PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.Storage conditions:-25°C?to?-18°CStorage instructions:Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.Recommended?Dilutions:IHC-P:?????1:100-1:200Background?References:1. ?Hirai,?H. et?al.?Novel?INK4?proteins,?p19 and?p18,are?specific ?inhibitors of the cyclin?D-dependent?kinases CDK4 and?CDK6.?Mol?Cell?Biol.?1995?May;?15(5):?2672–2681.2.?Sherr,?C.J.?Cancer?cell?cycles. Science.?1996?Dec 6;274(5293):1672-7.Immunohistochemistry?(Formalin/PFA-fixedparaffin-embeddedsections?analysis ofmantle?cell?lymphoma????labelling Cyclin?D1?with??BP6076.?Heat?mediated antigen?retrieval?wasperformed?usingTris/EDTA?buffer?pH?9.0. HKZ150194_BP6076_ CyclinD1下載 暫無 說明文檔下載說明書.pdf 上一篇: p120兔單克隆抗體下一篇: Cytokeratin 1兔單克隆抗體 推薦 ECL Basic（基本型） 2024年10月24日 1350 暫無 查看全文 鍍銀染液 2023年7月13日 1149 【送樣運(yùn)輸要求】 1.樣本放置于20倍樣本體積的固定液中固定24h以上，常溫運(yùn)輸送樣。 切勿固定時(shí)間過長，切勿冷凍結(jié)冰。 2.石蠟切片常溫運(yùn)輸 僅... 查看全文 Western Blot封閉液 2024年9月14日 1333 暫無 查看全文 內(nèi)源性過氧化物酶阻斷劑 2024年11月8日 1501 暫無 查看全文 您好！請登錄 點(diǎn)擊取消回復(fù) 請登錄后評論 點(diǎn)擊這里取消回復(fù)。
鍍銀染液 2023年7月13日 1149 【送樣運(yùn)輸要求】 1.樣本放置于20倍樣本體積的固定液中固定24h以上，常溫運(yùn)輸送樣。 切勿固定時(shí)間過長，切勿冷凍結(jié)冰。 2.石蠟切片常溫運(yùn)輸 僅... 查看全文
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