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Villin兔單克隆抗體

Villin兔單克隆抗體

  • 價(jià)格￥600
  • 規(guī)格100ul
  • 貨號HKZ150184
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150184Villin兔單克隆抗體100ul支600
Villin
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50061?Clone# ?BP6066
Predicted?Molecular Wt:
93kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P09327
Background:
Villinisa 93?kDa calcium-regulated, actin-binding?protein that?plays a?role?in?regulating?actin?filament?assembly.?It?is?a?major?constituent?in the?microvilli, which compose the?brush?border of epithelial?cells forming?absorptive?surfaces?of the?intestinal?and?renal?proximal tubular epithelia.
In?normal?human tissue, villin?is expressed?by a?limited?number of?simple?epithelia?of the?gastrointestinal?and?urogenital?tract.?In tumor tissue,?it?is always expressed?in colorectal?cancer,?hepatocellular?carcinoma,?endometrium?cancer,?as well?as?cancers??of ovary?and?lung.
Villinisa?useful aid for classification of?primary and?metastatic?colorectal?carcinomas.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to Villin?residues
within?aa600-700 of??Villin?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?R?Mollet?al.?Histochem?Cell?Biol?(2008)?129:705–733.
2. ?R?Mollet?al.?Cell, Vol.?31,?1?l-24,?November?1982.
Immunohistochemistry?(Formalin/PFA-fixedparaffin-embedded
sections?analysis of?colon?tissue?labelling Villin with?BP6066.?Heat?mediated ???antigen?retrieval was
performed?using
Tris/EDTA?buffer?pH?9.0

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