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COL4A3兔單克隆抗體

COL4A3兔單克隆抗體

  • 價(jià)格￥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150400
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150400COL4A3兔單克隆抗體100ul支1000
COL4A3
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50277?Clone# ?BP6254
Predicted?Molecular Wt:
162kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q01955
Background:
Six α chain of Type?IV collagen?can?aggregate?into?3?triple?helix?molecular?structure,?which?called?monomer,?monomer
polymerization to form adimer or tetramer, which?can twist?each?other?to?form?a?collagen?network?structure.?Mutations?in the X-linked dominant Alport comprehensive acquisition?gene?mainly?occurred?in the?gene?encoding?collagen?type?IV?ɑ5?chain?(COL4A5).?Mutations?in the autosomal?recessive Alport syndrome occur?on?chromosome?2?in genes?encoding?collagen?type?IV??ɑ3 or?ɑ4?strands?(COL4A3/COL4A4)?.COL4A3?is?mainly expressed?in?podocytes?in?Bowman’s capsule of glomeruli.
Subcellular?location:
Basement?membrane
Recommended?Method:
High temperature and?high?pressure?induced epitope?retrieval?with?Tris-EDTA?buffer?(pH?9.0),?primary
antibody?incubate?at?RT?(18°C-25°C) for?30?minutes.?Amplifier?for?Mouse?+?Rabbit?IgG?for?25min?，?HRP Polymer?Detector?for?25?min.
Immunogen:
Recombinant?protein fragment corresponding to???human?COL4A3. This?information?is?proprietary?to Biolynx and/or?its suppliers.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage Conditions:
-25°C?to?-18°C
Shipment?Instructions:
Shipped?on?blue?ice.?Upon?delivery?store?at?-25°C?to?-18?°C. Avoid?freeze?/ thaw?cycles.
Recommended?Dilution:
IHC-P:?????1:100-1:200
Background?References:
1.?Tsugio?Seki?et?al.?Histochem?Cell?Biol?(1998)?110:359-?366
2.?Yoshioka?K?etal. Am. J.?Pathol?(1994)?.?144: 986-996
Immunohistochemistry?(Formalin/PFA-fixed?paraffin-embedded?sections)?analysis of human?kidney?labelling?COL4A3 with?BP6254.

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