Ki-67兔單克隆抗體

價格￥600
規(guī)格100ul
貨號HKZ150327
單位支
品牌HaoKebio

產(chǎn)品介紹

產(chǎn)品參數(shù)

資料下載

品牌	商品編碼	商品名稱	規(guī)格	單位	單價
HaoKebio	HKZ150327	Ki-67兔單克隆抗體	100ul	支	600

Ki-67
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50204?Clone# ?BP6181
Predicted?Molecular Wt:
359kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P46013
Background:
Antigen?Ki-67?is a?nuclear?protein that?is associated with and?may?be?necessary?for?cellular?proliferation.?Furthermore,?it?is?associated with?ribosomal?RNA transcription.
Ki-67 was?present?in the?nuclei of cells?in the G1,S,and?G2?phases?of the?cell?division?cycle?as?well?as?in?mitosis.?Quiescent?or?resting?cells?in?the?G0?phase?did?not?express?the?Ki-67?antigen.
Ki-67?protein was?used as diagnostic tools?in?different types?of?neoplasms?because?it was?present?in?all?proliferating?cells
(normal and tumor cells) and was?an?excellent?operational?marker?to?determine?the?growth?fraction?of?a?given?cell?population.
Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within
aa1000 to?aa1100 of?Ki-67 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Scholzen?T, et.al, J?Cell?Physiol.?2000?Mar;182(3):311-?22.
2. ?Inwald?EC, et.al,?Breast?Cancer?Res?Treat.?2013?Jun;139(2):539-52.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of tonsil?labelling?Ki-67?with
BP6181.?Heat?mediated antigen?retrieval was
performed?using
Tris/EDTA?buffer?pH?9.0.

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