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Napsin A兔單克隆抗體

Napsin A兔單克隆抗體

  • 價格¥1000
  • 規(guī)格100ul
  • 貨號HKZ150201
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150201Napsin A兔單克隆抗體100ul1000
Napsin?A
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50078?Clone# ?BP6083
Predicted?Molecular Wt:
45kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??O96009
Background:
Napsin A?is a?pepsin-like aspartic?proteinase contains 420?amino?acids.?It?is?predominantly?expressed?in the?lung?and?kidney.?In?the?lung,?Napsin A?is expressed?in?alveolar?type?II?pneumocytes,?regulated?by TTF-1, and?is?involved?in the generation?of the
surfactant?protein?B.?Intra-alveolar?macrophages contain?Napsin A as a?result?of?phagocytosis.?In the?kidney,?Napsin?A?is?expressed?in the?proximal tubules,?where?it?is?involved?in?lysosomal?protein catabolism.
Napsin A?is?important?in the differential diagnosis of?lung?adenocarcinoma vs.?squamous?cell?carcinoma,?used?in?a?panel?with?TTF-1, CK5 and?p63.?For tumours?presenting as adenocarcinoma?of?unknown?origin,?the?identification?of?a?lung?origin?may?be?aided?by?NapA together with TTF1, and?renal origin?by?NapA together?with?PAX8.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within?aa50-150 of?Napsin?A?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?AgackiranY, Ozcan A, Akyurek?N,?Memis?L. Appl
Immunohistochem?Mol?Morphol.?2012 Jul;20(4):350-5.
2.?Chernock?RD,?El-Mofty?SK,?Becker?N,?Lewis?JS?Jr. Am?J Surg?Pathol.?2013?May?15.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of?lung?adenocarcinoma
labelling?Napsin A with???BP6083.?Heat?mediated antigen?retrieval?was
performed?using
Tris/EDTA?buffer?pH?9.0.

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