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CD34兔單克隆抗體

CD34兔單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150165
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150165CD34兔單克隆抗體100ul1000
CD34
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# BX50042?Clone# ?BP6047
Predicted?Molecular Wt:
41kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P28906
Background:
CD34?(also?named?myeloid?progenitor cell antigen)?is a?heavily glycosylated type?I?transmembrane?protein. There?are?two
forms of the CD34?protein,?resulting from alternative splicing. The?functions?of?CD34?is?largely?unknown,?but?recent?evidence?suggests a?role for?CD34?in cell adhesion?and?inhibition?of?hematopoiesis.?It?is suggested?that?CD34?is?a?signalling?molecule
involved?in?maintenance of a?phenotypically?plastic state?in?undifferentiated cells.
CD34?is found?in?most endothelia, expressed on the?luminal?surface?and?membrane?processes?interdigitating?between
endothelial cells,?but?is absent from?large veins and?arteries.?CD34?is furthermore?expressed?in fibroblast-like?dendritic?cells?in,?e.g.,?portal?tracts?of?the?liver,?Peyer’s?patches, and?in?healing?wounds.?In?smooth?muscle?cells,?a?variable?CD34?staining?is
found.?In tumor tissues, CD34?is detected?in?myeloid?blasts?in?myelodysplastic syndrome?and?acute?myeloid?leukaemia?in?most?cases as well as?lymphoblasts?in?most cases of?B-acute?lymphoblastic?leukaemia.?Mature?B-?and?T-cell?lymphomas?and
leukaemias are CD34?negative. The?majority of vascular tumours,?including?haemangiosarcoma?and?Kaposi?sarcoma?are?CD34?positive.
In a?panel,?CD34 staining?is?useful for the?classification?of?myeloid?and?lymphoid?neoplasms?as well?as?spindle?cell?neoplasms?(particularly?identification of gastrointestinal stromal tumour and?haemangiosarcoma).
Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to CD34?residues
within?aa285-385 of?CD34 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Baumheter?S, ?et.al,?Science.?1993?Oct 15;262(5132):436-8.
2. ?Fina?L, et.al,?Blood.?1990 Jun?15;75(12):2417-26.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of
kidney tissue?labelling
CD34 with?BP6047.?Heat mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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