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HER2兔單克隆抗體

HER2兔單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150138
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150138HER2兔單克隆抗體100ul1000
HER-2
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# ?BX50015?Clone# ?BP6020
?
Predicted?Molecular Wt:
138kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P04626

Background:
HER-2?(also?called?HER-2/neu, c-erbB2,?ERBB2 or?neu)?is?a?transmembrane?receptor?tyrosine?kinase,?which?is?a?proto-???oncogene,?i.e.?its activation causes?malignant transformation and?increases the?malignant?potential?(cell?proliferation,
invasiveness etc.) of the cells.?In?human cancers?HER-2?is?activated?via?gene?amplification,?which?is?a?genomic?mutation?where?a?small?fragment?at?chromosome?band?17q12-q21?is?multiplied?in?a?cell?up?to?50-100?folds.
?
HER-2?is?over-expressed?in?15-25% of?primary?breast?cancers, can?also?be?found?in?intestinal?type?gastric?and?gastroesophageal carcinomas, ovarian carcinomas,?high grade endometrial?carcinomas?and?some salivary?duct?tumors.?Low-level?copy?number?????increases?have?been found also?in?rare cases of?lung tumors.
?
Studies?have shown that c-erbB-2?positive?breast cancer?usually correlates?with?negative?staining?for?estrogen?and?progesterone?receptors; thus a?poorer?predictive outcome?is correlated with?positive?c-erbB-2?staining.
?

Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide?according?to?the?aa1155-1255?(C-?terminal) of ?HER-2?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
human?breast cancer
tissue?labelling?HER-2 with?BP6020.?Heat?mediated
antigen?retrieval was?performed?using
Tris/EDTA?buffer?pH?9.0
Background?References:
1.?Olayioye?M.A., et.al. Chem.?274:17209-17218(1999).
2.?Wang?S.C., et.al. Cancer?Cell?6:251-261(2004).

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