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Topoisomerase II-α兔單克隆抗體

Topoisomerase II-α兔單克隆抗體

  • 價(jià)格¥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150134
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150134Topoisomerase II-α兔單克隆抗體100ul1000
Topoisomerase?II-α
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# ?BX50011?Clone# ?BP6016
?
Predicted?Molecular Wt:
174kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P11388
?

Background:
The topoisomerase?II-α?isoformisa?170?kDa?nuclear?protein and?plays an?important?role?in?DNA synthesis and?RNA transcription,?as well as chromosomal segregation?during?mitosis.
?
Topoisomerase?II-α ?is?reported?to?be?a?sensitive?and?specific?marker?of?late?S-,?G2- &?M-phases?in?transformed?and developmentally?regulated?normal cells, and?has?been shown to?be?over-expressed?in?many?human?cancers.
?
Decreased expression of Topoisomerase?II-α?is the?predominant?mechanism of?resistance to several?chemotherapeutic?agents.
?

Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to Topo?II α?residues
within?aa1431-1531 of?Topo?II?α was?used?as?an immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Lynch?BJ, et?al,?Hum?Pathol.?1997 Oct;28(10):1180-8.
2.?Gupta?D, et al,Appl?Immunohistochem?Mol?Morphol.
2001 Sep;9(3):215-21.

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