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APOL1鼠單克隆抗體

APOL1鼠單克隆抗體

  • 價格¥1960
  • 規(guī)格100ul
  • 貨號HK510007L
  • 單位
  • 品牌HaoKebio

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HAOKEBIOHK510007LAPOL1鼠單克隆抗體100ul1960

Applications

Tested Applications:

FC, IF, IHC, IP, WB,ELISA


Cited Applications:

IF, IP, WB

Species Specificity:

human


Cited Species:

human


Positive WB detected in:

human plasma tissue


Positive IP detected in:

human plasma tissue


Positive IHC detected in:

human liver tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:

HepG2 cells


Positive FC detected in:

HepG2 cells


Recommended dilution:

WB : 1:20000-1:100000

IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB

IHC : 1:50-1:1000

IF : 1:50-1:500

Product Information


Source:

Mouse


Purification method:

Protein A purification


Isotype:

IgG2a


Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:

apolipoprotein L, 1


Calculated molecular weight:

44 kDa


Observed molecular weight:

39-45 kDa


Gene symbol

APOL1


Synonyms

APO L, APOL, APOL I, APOL1, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

Background

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.

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