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- 價(jià)格￥600
- 規(guī)格100ul
- 貨號(hào)HKZ150191
- 單位支
- 品牌HaoKebio
產(chǎn)品介紹
產(chǎn)品參數(shù)
資料下載
| 品牌 | 商品編碼 | 商品名稱 | 規(guī)格 | 單位 | 單價(jià) |
|---|
| HaoKebio | HKZ150191 | CD45兔單克隆抗體 | 100ul | 支 | 600 |
|---|
CD45
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# BX50068?Clone# ?BP6073
Predicted?Molecular Wt:
147kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P08575
Background:
CD45?is a family of single chain transmembrane?glycoproteins,?consisting?of?at?least?four?isoforms?which?share?a?common?large?intracellular domain. Their extracellular domains are?rod?shaped,?heavily?glycosylated. The?different?isoforms?are?produced?by??alternative?messenger?RNA?splicing?of?three?exons?of?a?single?gene?on?chromosome?1q31-32.
CD45?is exclusively expressed?in?haematolymphoid cells. Almost?all?haematolymphoid cells,?including?precursor?cells?and
mature?B- and T-lymphocytes, granulocytes,?monocytes/histiocytes and?interdigitating?reticulum cells and?follicular?dendritic
cells express CD45. This?protein?is detected?in the?large?majority?of?haematolymphoid?neoplasms,?i.e.,?leukemias and?malignant?lymphomas. Overall, about?90% of?malignant?lymphomas?are?CD45?positive.
CD45?is an?important?marker?in the?primary tumor screening?panel?in order to?identify?haematolymphoid?differentiation.?Loss?of CD45?in?precursor?B-cell?neoplasms?is a?negative?prognostic?parameter.
Subcellular?location:Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Recombinant?fragment?within?Human?CD45 aa?1 to aa600.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Dahlke?MH?etal.?Leuk?Lymphoma.?2004?Feb;45(2):229-?36.
2.?Gaertner?EM??etal. Am?J?Clin?Pathol.?2001?Apr;115(4):589-97.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of tonsil?tissue?labelling?CD45
with?BP6073.?Heat mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0
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