PMEL17兔單克隆抗體

價格￥600
規(guī)格100ul
貨號HKZ150149
單位支
品牌HaoKebio

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產(chǎn)品參數(shù)

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品牌	商品編碼	商品名稱	規(guī)格	單位	單價
HaoKebio	HKZ150149	PMEL17兔單克隆抗體	100ul	支	600

PMEL?17
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# ?BX50026?Clone# ?BP6031
?
Predicted?Molecular Wt:
70kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P40967
?

Background:
PMEL?17?isa?melanosome specific antigen, also?is designed?as?HMB45?or gp100.?It?plays?acentral?role?in?the?biogenesis?of
melanosomes,?involved?in the?maturation of?melanosomes from stage?I to?II. The transition?from?stage?I?melanosomes to?stage?II?melanosomes?involves an elongation of the vesicle, and?the?appearance?within?of?distinct?fibrillar?structures.
PMEL?17?is?presented?in?immature and activated?melanocytes?(due to, e.g.,?inflammation,?increased vascularity?or?underlying?tumor),?but?not?in?mature,?resting?melanocytes.?In tumor tissues,?it?is?restricted expressed?in?melanoma.
?Studies?support?the?routine?use?of?PMEL?17?(HMB45) as?a?sensitive?and?specific?melanocytic?marker.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?PMEL?17?residues
within?aa561‐661 of?PMEL?17 was?used?as?an immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections?analysis of?human?melanoma tissue?labelling??PMEL?17 with?BP6031.
Heat?mediated?antigen
retrieval?was?performed????using Tris/EDTA?buffer?pH?9.0
Background?References:
1. ?Rothberg?BE, et.al,?Mod?Pathol.?2008 Sep;21(9):1121-9.
2.?Wagner?SN, et.al, Cancer?Immunol?Immunother.?1997?Jun;44(4):239-47.

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