細(xì)胞角蛋白廣譜兔單克隆抗體

品牌	商品編碼	商品名稱(chēng)	規(guī)格	單位	單價(jià)
HaoKebio	HKI0811A	細(xì)胞角蛋白廣譜兔單克隆抗體	100ul	支	975

Applications

Tested Applications:

FC, IF, IHC, IP, WB,ELISA

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Cited Applications:

IF, IHC, WB

Species Specificity:

human, mouse

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Cited Species:

human, mouse

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Positive WB detected in:

A431 cells, HEK-293 cells, HeLa cells, mouse skin tissue

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Positive IP detected in:

A431 cells

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Positive IHC detected in:

human cervical cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

HeLa cells

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Positive FC detected in:

HeLa cells

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Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:50-1:500

IF : 1:50-1:500

Product Information

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Source:

Rabbit

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Purification method:

Antigen affinity purification

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Isotype:

IgG

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Immunogen:

Peptide

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Full name:

keratin 17

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Calculated molecular weight:

48 kDa

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Observed molecular weight:

48 kDa

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Gene symbol

KRT17

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Synonyms

39.1, CK 17, Cytokeratin 17, K17, keratin 17, KRT17, PCHC1

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Protocols

PRODUCT