EGFR（ L858R)兔單克隆抗體

價格￥2200
規(guī)格100ul
貨號HKZ150216
單位支
品牌HaoKebio

產(chǎn)品介紹

產(chǎn)品參數(shù)

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品牌	商品編碼	商品名稱	規(guī)格	單位	單價
HaoKebio	HKZ150216	EGFR（ L858R)兔單克隆抗體	100ul	支	2200

EGFR（?L858R）
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50093?Clone# ?BP6098
Predicted?Molecular Wt:
134kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P00533
Background:
Receptor tyrosine?kinase?binding?ligands of the?EGF family and activating?several?signaling?cascades to?convert?extracellular?cues?into appropriate cellular?responses.?Known?ligands?include?EGF, TGFA/TGF-alpha,amphiregulin, epigen/EPGN,
BTC/betacellulin, epiregulin/EREG and?HBEGF/heparin-binding?EGF.?Ligand?binding triggers?receptor?homo- and/or
heterodimerization and autophosphorylation on?key cytoplasmic?residues. The?phosphorylated?receptor?recruits?adapter
proteins?like GRB2 which?in turn activates complex?downstream?signaling?cascades. Activates?at?least?4?major?downstream
signaling cascades?including the?RAS-RAF-MEK-ERK,?PI3?kinase-AKT,?PLCgamma-PKC and STATs?modules.?May also?activate?the?NF-kappa-B signaling cascade. Also directly?phosphorylates other?proteins?like?RGS16,?activating?its?GTPase?activity?and
probably coupling the?EGF?receptor signaling to the G?protein-coupled?receptor?signaling.?Also?phosphorylates?MUC1?and
increases?its?interaction with SRC and CTNNB1/beta-catenin.?Plays?a?role?in?enhancing?learning?and?memory?performance?(By?similarity).
The?antibody?is?specific?expressed?on?EGFR(L858R) mutated?lung?cancer.
Subcellular?location:
Cytomembrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer??(pH?9.0),?primary?antibody?incubate?at?4°C?overnight.
Immunogen:
A synthetic?peptide corresponding to?EGFR?residues?within?aa800-900 of??EGF?receptor?(mutated?L858R)?was?used as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Koo?JS, et?al.?Neoplasma.?2011;?58(1):27-34.
2. ???Lu?X,Yu?L, et?al.??MedResRev2018;38:1550–1581.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of
lung carcinoma??tissue?(mutated?L858R)
labelling?EGFR?(L858R)?with?BP6098.?Heat
mediated?antigen
retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

HKZ150216_BP6098_ EGFR(L858R)下載

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