TPO Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150066 Clone# BP6071 Predicted Molecular Wt: 103kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P07202 Background: Thyroid peroxidase (TPO) is present as a dimer on the apical surface of thyroid follicular cells, and it is the primary enzyme involved in thyroid hormone synthesis. TPO can be found in normal thyroid and thyroid carcinoma of papillary and follicular type, and it is a useful marker for the differentiation of benign and malignant thyroid neoplasms. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to TPO residues within aa833-933 of TPO was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. De Micco C et al. Eur J Endocrinol 1994;131:474-9. 2. De Micco C et al. Thyroid 2000;10:109-15. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of thyroid tissue labelling TPO with BP6071. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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